Fibronectin Printing for Stem Cell Adhesion

Surface Patterning by Micro-Contact-Printing

Human Umbilical Cord Blood Neural Stem Cells (HUCB-NSC) were grown on microcontact printed fibronectin squares for a differentiation study.

The squares were printed using PDMS stamp on a cell repellent, plasma polymerized poly-ethylene-oxide (PEO) like surface using fibronectin solution in an acetate buffer as an ink.

Fibronectin pattern

HUCB-NSC cells on micro-contact printed Fibronectin squares – Phase contrast image

Fluorescence Image - Green: β-tubulin-II neuronal marker; Red: GFAP; Blue: Cell nuklei

Fluorescence Image – Green: β-tubulin-II neuronal marker; Red: GFAP; Blue: Cell nuklei

 

 

 

 

 

 

 

 

 

 

The cells do not adhere to the PEO coated regions, but are attached to the printed 100 µm fibronectin squares. After the culturing period the cells were fixed and immunostained for glial and neuronal differentiation markers.


Courtesy of EC Joint Research Centre, Nanobiosciences Unit, Ispra, Italy (Dora Mehn)