Advanced Tools for BS3.1
Dec. 2016: We are proud to annouce new printing tools for the BioScaffold Printer BS3.1. Melt Electrospinning Writing allows the accurate deposition of polymer fibres below 20 Micrometer diameter.
The Pneumatic Core/Shell Extruder combines biopolymers of different characteristics, e.g. e soft polymer hosting living cells in the core lane with a more rigid biopolymer in the shell lane.
Enridgement and Manipulation of Microorganisms
Oct 2016: In collaboration with IBA Heiligenstadt e.V. GeSiM developed a customized MicCell to investigate microorganisms in drinking water. This MicCell comprises three fluidic channels. Cell manipulation is achieved by interdigital electrodes with sizes from 20…100 Micrometers. We embedded up to 12 different IDEs for
- Dielectrophoretic enridgement
- Pulsation based perforation of cell membranes
- Measurement of conductivity changes
Meet us at upcoming events!
Sept. 2016: There are a few events coming up dealing with state-of-the art aspects of tissue engineering and 3D printing:
- The annual conference of the German Society for Biomaterials will take place on 29th September to 1st October in Aachen. We will demonstrate the latest BioScaffold printer BS3.1
- A bioprinting and 3D printing conference to be held in Cambridge, UK (13/14 October). Our UK distributor, Analytic Ltd., will man a booth.
Don’t miss these opportunities to meet up with GeSiM, We look forward to fruitful talks with you.
We welcome Neutec Group Inc!
May 2016: GeSiM is proud to announce the partnership with Neutec Group Inc., Farmingdale, NY.
The established sales company will start to promote the new GeSiM BioScaffoldprinter BS3.1 within the territory of North America. It allows customers in the US and Canada to purchase Bioprinter products and accessories from a local source.
New Demo Lab in Shanghai
May 2016: GeSiM is now preparing new lab space in one of Asia’s leading Biotech Hotspots. It will allow local prospects to see and try GeSiM instruments before doing an investment.
The lab is supposed to open mid July and will be managed by our Asian Sales Manager. It is located in the Baoshan District of Shanghai. Please contact us for detailed information.
Currently there are no vacant positions at GeSiM mbH. Please revisit this page from time to time.
Low-cost flexible electronics with organic transistors calls for an easy and cost-effective patterning process. While contact printing, e.g. using reels, might be useful for large-scale production, ink-jet printing ensures high flexibility.
P3OT has been extensively studied as soluble organic semiconductor, but mostly in chloroform solutions. We tested the printing of organic field-effect transistors (oFETs) using different inks:
- Commercial poly-(3-octylthiophene-2,5-diyl) (P3OT), 1 – 3 mg/ml in different solvents
- Chloroform, chlorobenzene, trichloroethylene, xylene
- Back side-metallized doped Si as gate
- 140 nm thermal oxide as gate dielectrics
- 150 nm Ti/Au lift-off-patterned S/D-electrodes yielding 20-50 µm x 1-3 mm transistor channels
- Rinsing in chloroform immediately before printing to obtain hydrophobic surfaces
- Nano-PlotterTM , XYZ robotic stage with microwell plate for inks and washing solvents
- Piezoelectrically driven Nano-Tip fabricated by Si-micromechanics with tip-outlet to reduce wetting
- Camera system for function control of jetting and adjustment to substrate
- Normal atmosphere (extendable to housed processing under nitrogen o.a.)Micro-Dosage of P3OT
Micro-Dosage of P3OT
- Printing only possible from solutions with higher boiling points (not chloroform)
- Line printing by overlapping of single dots
- Problems (flake-like overlap) with trichloroethylene-based solution because of fast evaporation
- Good printing from chlorobenzene and xylene solutions
- Spot diameter ~ 150 – 180 µm, depending on concentration and jetting parameters
We prepared oFETs with common Si/SiO2-Au bottom contact design using ink-jet printed semiconductor lines. To reduce evaporation, high-boiling solvents were used. We could print P3OT in trichloroethylene, chlorobenzene, and xylene. The printed oFETs revealed Ion/Ioff-ratios up to 20,000 and hole mobilities of up to 0.002 cm²/Vs (xylene solution). Although this does not represent ideal data, the ease of patterning offers a route to low-cost electronics. It also inspires to study patterning of other semiconducting materials with drastically decreased gate leakage. Equipment: Nano-Plotter NP2.0
Peptides are used by nature as active regulators and messengers in the human body. They combine high specificity with high affinity in molecular recognition processes, therefore they are ideally suited as drugs. Peptide microarrays can be utilized for the identification of peptide sequences suitable for pharmaceutical or diagnostic applications as well as for the screening of enzymatic substrates or for enzyme inhibitors. In a passed research project the Nano-Plotter is applied to the automated synthesis of peptide libraries on a variety of different solid supports such as glass microcapillary plates. The spatially addressable synthesis of different peptides on solid supports allows the subsequent screening of the peptide libraries by established assay techniques including optical screening by fluorescence methods. Only very few peptide sequences from a combinatorial library are typically suitable for the desired tasks, therefore it is highly desirable to produce only the minimal quantity of the candidate sequences necessary for screening. This saves costs and is environmentally friendly. For the realization of microreactions the Nano-Plotter is particularly suited, as it is capable to perform dosage steps in the microliter as well as in the nanoliter range. These dosage steps can be performed highly reproducibly at desired reaction coordinates in the nanoliter scale, a precondition which is indispensable for the generation of peptide arrays in the biochip format on glass surfaces.
Using the Nano-Plotter two different approaches in the generation of peptide libraries on glass biochips are possible. On one hand “classical” replica peptide libraries can be generated by spotting solutions of preassembled peptides onto modified glass surfaces. Such peptide libraries for example are commercially available in microtiter plates, the peptides have to be equipped with special linker groups which allow their covalent attachment to the glass surface. Using this technique, the Nano-Plotter is capable of producing a high number of identical copies of given peptide libraries for screening purposes.
On the other hand, for the first time, the Nano-Plotter allows the sequential on-chip synthesis of peptides from activated amino acid building blocks utilizing the Fmoc-strategy. In this setup solutions of activated Fmoc amino acid building blocks are generated in situ by the Nano-Plotter and are spotted onto separate synthesis areas on glass microcapillary plates. This way, picomolar amounts of up to 900 different peptides are synthesized at specified positions on a glass microcapillary plate which can then directly be used in optical screening experiments. The wash- and deprotection steps required in this procedure are performed automatically in a novel, chemically inert GeSiM synthesis chamber. With this approach it is possible to produce unique peptide libraries consisting of custom peptides, therefore it provides the highest flexibility concerning the diversity of candidate sequences.
Equipment: Nano-Plotter NP2.0/E