Category Archives: Allgemein

Products & Applications

The Nano-Plotter is ideal to generate high-quality spots by non-contact microdispensing of sub-nanoliter volumes.

  • Array based Diagnostics
  • Organic Light Emitter Diodes (OLEDs)
  • Microdispensing of functional liquids is an essential step in
  • production of organic electronics
  • Reverse Phase Microarrays
  • Immunoassay for Allergy Diagnostics
  • Printing of Organic Field Effect Transistors
  • Arraying into 96-well Plates
  • Array Calibration for Antibody Spotting
  • Peptide Synthesis Project
  • Glycoprotein Arrays
  • Coating of Drug Eluting Stents

Ink-Jet Printing of Organic Field-Effect Transistors

Low-cost flexible electronics with organic transistors calls for an easy and cost-effective patterning process. While contact printing, e.g. using reels, might be useful for large-scale production, ink-jet printing ensures high flexibility.

P3OT has been extensively studied as soluble organic semiconductor, but mostly in chloroform solutions. We tested the printing of organic field-effect transistors (oFETs) using different inks:

Semiconductor:

  • Commercial poly-(3-octylthiophene-2,5-diyl) (P3OT), 1 – 3 mg/ml in different solvents

Solvents:

  • Chloroform, chlorobenzene, trichloroethylene, xylene

Test vehicles:

  • Back side-metallized doped Si as gateBildschirmfoto 2014-09-02 um 16.09.26
  • 140 nm thermal oxide as gate dielectrics
  • 150 nm Ti/Au lift-off-patterned S/D-electrodes yielding 20-50 µm x 1-3 mm transistor channels
  • Rinsing in chloroform immediately before printing to obtain hydrophobic surfaces

 

Ink-jet printer:

  • Nano-PlotterTM , XYZ robotic stage with microwell plate for inks and washing solvents
  • Piezoelectrically driven Nano-Tip fabricated by Si-micromechanics with tip-outlet to reduce wetting
  • Camera system for function control of jetting and adjustment to substrate
  • Normal atmosphere (extendable to housed processing under nitrogen o.a.)Micro-Dosage of P3OT

Micro-Dosage of P3OT

  • Printing only possible from solutions with higher boiling points (not chloroform)
  • Line printing by overlapping of single dots
  • Problems (flake-like overlap) with trichloroethylene-based solution because of fast evaporation
  • Good printing from chlorobenzene and xylene solutions
  • Spot diameter ~ 150 – 180 µm, depending on concentration and jetting parameters

Bildschirmfoto 2014-09-02 um 16.11.02

Summary

We prepared oFETs with common Si/SiO2-Au bottom contact design using ink-jet printed semiconductor lines. To reduce evaporation, high-boiling solvents were used. We could print P3OT in trichloroethylene, chlorobenzene, and xylene. The printed oFETs revealed Ion/Ioff-ratios up to 20,000 and hole mobilities of up to 0.002 cm²/Vs (xylene solution). Although this does not represent ideal data, the ease of patterning offers a route to low-cost electronics. It also inspires to study patterning of other semiconducting materials with drastically decreased gate leakage.   Equipment: Nano-Plotter NP2.0

Development of a Pipetting Station for the Parallel Synthesis

Peptides are used by nature as active regulators and messengers in the human body. They combine high specificity with high affinity in molecular recognition processes, therefore they are ideally suited as drugs.   Peptide microarrays can be utilized for the identification of peptide sequences suitable for pharmaceutical or diagnostic applications as well as for the screening of enzymatic substrates or for enzyme inhibitors.   In a passed research project the Nano-Plotter is applied to the automated synthesis of peptide libraries on a variety of different solid supports such as glass microcapillary plates. The spatially addressable synthesis of different peptides on solid supports allows the subsequent screening of the peptide libraries by established assay techniques including optical screening by fluorescence methods. Only very few peptide sequences from a combinatorial library are typically suitable for the desired tasks, therefore it is highly desirable to produce only the minimal quantity of the candidate sequences necessary for screening. This saves costs and is environmentally friendly. For the realization of microreactions the Nano-Plotter is particularly suited, as it is capable to perform dosage steps in the microliter as well as in the nanoliter range. These dosage steps can be performed highly reproducibly at desired reaction coordinates in the nanoliter scale, a precondition which is indispensable for the generation of peptide arrays in the biochip format on glass surfaces. Bildschirmfoto 2014-09-02 um 14.20.34

Using the Nano-Plotter two different approaches in the generation of peptide libraries on glass biochips are possible. On one hand “classical” replica peptide libraries can be generated by spotting solutions of preassembled peptides onto modified glass surfaces. Such peptide libraries for example are commercially available in microtiter plates, the peptides have to be equipped with special linker groups which allow their covalent attachment to the glass surface. Using this technique, the Nano-Plotter is capable of producing a high number of identical copies of given peptide libraries for screening purposes.
On the other hand, for the first time, the Nano-Plotter allows the sequential on-chip synthesis of peptides from activated amino acid building blocks utilizing the Fmoc-strategy. In this setup solutions of activated Fmoc amino acid building blocks are generated in situ by the Nano-Plotter and are spotted onto separate synthesis areas on glass microcapillary plates. This way, picomolar amounts of up to 900 different peptides are synthesized at specified positions on a glass microcapillary plate which can then directly  be used in optical screening experiments. The wash- and deprotection steps required in this procedure are performed automatically in a novel, chemically inert GeSiM synthesis chamber. With this approach it is possible to produce unique peptide libraries consisting of custom peptides, therefore it provides the highest flexibility concerning the diversity of candidate sequences.

Bildschirmfoto 2014-09-02 um 15.25.46

Equipment: Nano-Plotter NP2.0/E

Arraying of PEDOT:PSS for Organic Light Emitter Diodes (OLEDs)

Microdispensing of functional liquids is an essential step in production of organic electronics and organic light emitter diodes (OLED). Piezoelectric printing is widely used to build up circuits and arrays of transistors and of optical devices. The GeSiM drop-on-demand dispensers work well with a variety of organic and anorganic liquids. This application note outlines the results for: Poly(styrenesulfonate)/poly(2,3-dihydrothieno(3,4-b)-1,4-dioxin) 2.8 wt% in H2O [Aldrich 560596-25G], also known as PEDOT:PSS. PEDOT:PSS is required for the hole-injection and transportation layers of the OLED-pixels. Arrays of different formats, differently sized spots and different densities are available with the piezoelectric micropipetting system Nano-Plotter . The surface tension of the substrate affects the spot diameter. Bildschirmfoto 2014-09-01 um 12.19.49 Dispensing of multiple drops allows a wide range of spot diameters with each dispenser but the single-drop volume with PEDOT:PSS depends both on the dispenser type and the dispense settings. The diagrams show the single-drop volume for piezoelectric Nano-Plotter dispensers (Pico-Tip, Nano-Tip, Nano-Tip-A) and for the standalone dispenser SPIP. The new LD04 is still under development. Bildschirmfoto 2014-09-01 um 12.23.21 Equipment: Nano-Plotter NP2.1 with different piezoelectric dispensers

Testbild News

16. June 2014

Donut-shaped chambers

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Testbild News

1 June 2014

Nanofair 2014

Nanofair 2014 will provide a forum for presenting current research and for the exchange of ideas and information between researchers, scientists and engineers from industry, research laboratories and academia. Contributions will cover fundamental scientific aspects as well as application oriented research and development. Furthermore successful implementations of new products using nanotechnology will be addressed as well. Continue reading

Nano-Plotter – Removable Slide Tray

Removable Slide Tray

For fast and easy replenishment of targets. A coolable slide tray slows down spot evaporation for higher spot quality.
Different layouts (coolable/non-coolable, with/without locator pins, for slides/MTPs, customized) available for both
NP 1.2 and NP 2.1.

 

nanoplotter-slide-deck-chilled-platform-gesim-1 Nanoplotter - a coolable slide tray slows down spot evaporation for higher spot quality

Nano-Plotter – Microplate Handler

Microplate Handler

Nanoplotter- microplate handler Cytomatn c425

The  Nano-Plotter (NP2.x only) hooks up to the plate hotel Cytomat 2 C425® (ThermoFisher Scientific). The plate hotel is humidity and temperature conditioned and deliveres up to 42 plates on request, thus enabling unattended runs of a large set of samples.

 

 

Nano-Plotter – Chilling and Humidifying

Chilling and Humidifying

These components are to tune the operational environment of slides and source plates. Evaporational losses can weaken the results of microarray experiments.

 

The ultrasonic humidifier blows an aerosol into the dust protection cover of the Nano-Plotter. The sensor controlled add-on unit increases the humidity smoothly up to 80%.

 

The recirculation bath (another add-on unit) connects to the slide deck as well as to the holder of the micro plate.

 

Nanoplotter Chiller nanoplotter-humidifiers-gesim nanoplotter-mtp-holder-gesim