Category Archives: Configurations

BioSyntheSizer

Robotic Multi-Organ-Chip Lab

Microfluidic Multi Cell Cultivation with automatic Sample Handling

The BioSynthesizer technology now fits to a robot called 2-level-Lab BSys 4.2 with elevated work deck. It is made to operate small transparent bio containments, e.g. SBS well format objects, microfluidic cartridges and others. It features two tool heads for optical inspection in both directions. The upper tool head operates all GeSiM made tools for liquid handling, Nanolitre pipetting and paste dispensing. The modular approach of all GeSiM instruments enables even 3D printing. Microfluidic cartridges and chips can be provided by GeSiM but this instrument also accepts customer designs.

The 2-level-lab robot is always configured to a particular application. It was designed for unattended run of complex sequences in cell labs, e.g. printing and cultivation of 3D organoids with one setup.

BLab Twin Head work deck with multi-organ-chips and 96well plates (middle)

2-level-Lab work deck with multi-organ-chips and 96well plates (middle)

This example accommodates an array of Multi-Organ-Chips (MOCs) on the left tray. Each MOC hooks up to a separate control unit for media and temperature adjustment. The MOC design can be adapted to different applications.

Examples of microfluidic chips for the 2-level-lab: Systemic toxicity assay (left), Hypoxia assay (right)

Examples of microfluidic chips for the 2-level-lab: Systemic toxicity assay (left), Hypoxia assay (right)

Sytemic toxicity testing

  • Two parallel-connected cell culture chambers (3), reservoir (2)
  • 3-point-peristaltic-pump (1), inlet and outlet valves (4)
  • Temperature controlled support

 

Hypoxia Assay

  • Oxygen control directly on culture media
  • External gas mixer
  • Gas permeable membrane for in/output

 

Please contact us with your specific idea.

 

Synthesis of Hydrogels

The Promise of Water containing Hydrogels

Hydrogels are widely used in regenerative medicine. The soft and water containing polymers are well appropriated as cell culture media but keep cells “in shape”: 3D printing arranges the hydrogel in a spatial manner or allows combined printing with other polymers.

In collaboration with the Leibniz Institute for Polymer Research Dresden a configuration for Hydrogel synthesis was developed. It starts with the conjugation of Polyethylene Glycol (PEG) with crysteine-containing peptides such that the conjugate contains a terminal thiol group. At the same time, heparin is coupled with reactive groups, all in an automated way. The PEG-peptide-thiol polymer is then linked to the heparin via a sulphur bridge, and other components (cells, adhesive peptides) are added. Star-hydrogels are possible.


The GeSiM BioScaffold printer is well appropriated for 3D prints with hydrogels. Please visit the related product site for BS3.1.