Proof-of-concept for automated synthesis of PET-Tracers
This tab outlines the result of a cooperative development of ABX GmbH and GESIM mbH, initiated by a BMBF supported joint research project (FKZ: 13N10271).
To conduct a PET scan, a short-lived radioactive tracer isotope is injected into the living subject (usually into blood circulation). The tracer is chemically incorporated into a biologically active molecule in order to fit a particular medical indication.
The tracer isotope cannot be produced in advance but must be synthesized shortly before the PET scan. On the other hand, the manipulation of radioactive components requires stringent work protection measures for the clinical staff.
BSyS 3.1 allows the unattended synthesis of multiple radioactive tracers for different medical indications. It brings up productivity of the PET facility to a new level:
- Up to eight validated synthesis per day
- Novel synthesis procedure established for [18F]FDG, FLT, FMISO, FNaF, FES, [18F]FET, FSB-peptides and [68Ga]GA-peptides
- 8 Kit plates featuring reactor, reagent and SPE purification and separate canulla reservoirs
This version of BSyS 3.1 still needs to be evaluated and certified for use with PET scanners in a clinical environment. Research groups from Roskilde and Copenhagen, Denmark, have used the BSyS 3.1 to develop Ti-containing cytotoxic compounds without cisplatin cross-resistance. However, at present no protocol exists that is working out of the box.
The Promise of Water containing Hydrogels
Hydrogels are widely used in regenerative medicine. The soft and water containing polymers are well appropriated as cell culture media but keep cells “in shape”: 3D printing arranges the hydrogel in a spatial manner or allows combined printing with other polymers.
In collaboration with the Leibniz Institute for Polymer Research Dresden a configuration for Hydrogel synthesis was developed. It starts with the conjugation of Polyethylene Glycol (PEG) with crysteine-containing peptides such that the conjugate contains a terminal thiol group. At the same time, heparin is coupled with reactive groups, all in an automated way. The PEG-peptide-thiol polymer is then linked to the heparin via a sulphur bridge, and other components (cells, adhesive peptides) are added. Star-hydrogels are possible.
The GeSiM BioScaffold printer is well appropriated for 3D prints with hydrogels. Please visit the related product site for BS3.1.