Category Archives: Applications

µContactPrinter

Grids of Microwells by NIL

Picoliter Wells by NIL

This example of an Israeli customer shows honeycomb shaped picowells at 150 microns size. They were done by nanoimprint lithography (NIL). They consist of UV curable photoresist. Immersed in a cell culture media these structures accomodate a single cell in each cavity.

 

Fibronectin Printing for Stem Cell Adhesion

Surface Patterning by Micro-Contact-Printing

Human Umbilical Cord Blood Neural Stem Cells (HUCB-NSC) were grown on microcontact printed fibronectin squares for a differentiation study.

The squares were printed using PDMS stamp on a cell repellent, plasma polymerized poly-ethylene-oxide (PEO) like surface using fibronectin solution in an acetate buffer as an ink.

Fibronectin pattern

HUCB-NSC cells on micro-contact printed Fibronectin squares – Phase contrast image

Fluorescence Image - Green: β-tubulin-II neuronal marker; Red: GFAP; Blue: Cell nuklei

Fluorescence Image – Green: β-tubulin-II neuronal marker; Red: GFAP; Blue: Cell nuklei

 

 

 

 

 

 

 

 

 

 

The cells do not adhere to the PEO coated regions, but are attached to the printed 100 µm fibronectin squares. After the culturing period the cells were fixed and immunostained for glial and neuronal differentiation markers.


Courtesy of EC Joint Research Centre, Nanobiosciences Unit, Ispra, Italy (Dora Mehn)

 

Nanolitre Wells with Electrode Patterns

NIL for in vitro Neurotoxicity Assay Development

Electrodes of the MEA chip in the bottom of the 100 x 100 x 50 µm wells

Electrodes of the MEA chip in the bottom of the 100 x 100 x 50 µm wells

Microelectrode array chambers (MEA chips) were applied as a support to generate 3D microwell environment for neural cells in electrical activity measurement studies.

The PVM -A combined with UV illumination and the 24 well stamp bodies were used to generate imprints with 100 µm sized wells and interconnecting channels aligned to the electrodes of the multi-electrode arrays. The matrix of PEG based hydrogel was polymerized by 60 s UV treatment.

 

 

Scanning electron microscopic image of one well of the microarray (by Cesar Pasqual Garcia)

Scanning electron microscopic image of one well of the microarray (by Cesar Pasqual Garcia)

Primary (rat) neurons grown forming a network in the microwells; Green: β-tubulin-II neuronal marker, Blue: cell nuclei. (by Jakub Nowak)

Primary (rat) neurons grown forming a network in the microwells; Green: β-tubulin-II neuronal marker, Blue: cell nuclei. (by Jakub Nowak)

 

 

 

 

 

 

 

 

 

 


Courtesy of EC Joint Research Centre, Nanobiosciences Unit, Ispra, Italy (Dora Mehn)