Cell Lysate Microarrays (Reverse Microarrays) – From Cells to Protein Profiles

Reverse Phase Protein Arrays

Microarrays with individual cell lysate spots of a 130 µm diameter, representing 1 to 10 cell equivalents each, are being produced using the robust piezo-electric non-contact deposition method of the Nano-Plotter. Signals are generated by fluorescently labeled antispecies antibodies. Due to the extremely low sample consumption of the spotting process large numbers of replicate arrays can be produced and thus allow the efficient determination of substantial numbers of proteins.  The simple, robust and highly parallel architecture of the assay yields precise quantitative information and allow time course monitoring of protein expression and activation.

From tissue to data (Source: Zeptosens AG)

From tissue to data (Source: Zeptosens AG)


From tissue to data: The procedure of Zeptosens Cell Lysate Microarrays







The applicability of the ZeptoMARK CeLyA approach has been demonstrated among others by

  • Identification of disease relavent marker proteins in cultured cells, tissues, microdissected material as well as depleted serum and urine
  • Determination of dose efficacy and IC50 of drug candidates
  • Quantification of cell signalling pathway activation / inhibition with a precision of better than 20%
  • Monitoring phosphorylation changes on multiple kinases

More information on this technology ist available from Bayer Technology Services, Leverkusen (German)