Nanolitre Wells with Electrode Patterns

NIL for in vitro Neurotoxicity Assay Development

Electrodes of the MEA chip in the bottom of the 100 x 100 x 50 µm wells

Electrodes of the MEA chip in the bottom of the 100 x 100 x 50 µm wells

Microelectrode array chambers (MEA chips) were applied as a support to generate 3D microwell environment for neural cells in electrical activity measurement studies.

The PVM -A combined with UV illumination and the 24 well stamp bodies were used to generate imprints with 100 µm sized wells and interconnecting channels aligned to the electrodes of the multi-electrode arrays. The matrix of PEG based hydrogel was polymerized by 60 s UV treatment.

 

 

Scanning electron microscopic image of one well of the microarray (by Cesar Pasqual Garcia)

Scanning electron microscopic image of one well of the microarray (by Cesar Pasqual Garcia)

Primary (rat) neurons grown forming a network in the microwells; Green: β-tubulin-II neuronal marker, Blue: cell nuclei. (by Jakub Nowak)

Primary (rat) neurons grown forming a network in the microwells; Green: β-tubulin-II neuronal marker, Blue: cell nuclei. (by Jakub Nowak)

 

 

 

 

 

 

 

 

 

 


Courtesy of EC Joint Research Centre, Nanobiosciences Unit, Ispra, Italy (Dora Mehn)