Publications & Resources

GeSiM bioinstruments and microfluidics are mostly placed into a scientific environment. We are proud to present customer projects conducted with our products. For further questions on the listed publications please contact us.


Bioprinting Access Authors/ Publication Date
Composite Bioinks With Mesoporous Bioactive Glasses—A Critical
Evaluation of Results Obtained by In Vitro Experiments
frontiers in Bioengineering and Biotechnology Vera Guduric, Johannes Wieckhusen, Anne Bernhardt, Tilman Ahlfeld, Anja Lode,
Chengtie Wu and Michael Gelinsky/ 11th January 2022
Core–shell bioprinting as a strategy to apply differentiation factors
in a spatially defined manner inside osteochondral tissue substitutes
 IOP Publishing Ltd. David Kilian, Silvia Cometta, Anne Bernhardt, Rania Taymour, Jonas Golde, Tilman Ahlfeld, Julia Emmermacher, Michael Gelinsky and Anja Lode/ 6th January 2022
Controlled and Local Delivery of Antibiotics by 3D Core/Shell Printed Hydrogel Scaffolds to Treat Soft Tissue Infections Pharmaceutics 2021 Ashwini Rahul Akkineni, Janina Spangenberg, Michael Geissler, Saskia Reichelt, Hubert Buechner, Anja Lode and Michael Gelinsky/ 14th December 2021
Electrostatic Distortion of Melt-Electrowritten Patterns by 3D Objects: Quantification, Modeling, and Toolpath Correction Advanced Materials Technologies Cathal D. O’Connell, Olivia Bridges, Cameron Everett, Natasha Antill-O’Brien, Carmine Onofrillo, Claudia Di Bella/ 15th July 2021
3D printing of patient‑specific implants for osteochondral defects:
workflow for an MRI‑guided zonal design
Bio-Design and Manufacturing David Kilian, Philipp Sembdner, Henriette Bretschneider, Tilman Ahlfeld, Lydia Mika, Jörg Lützner3, Stefan Holtzhausen, Anja Lode, Ralph Stelzer, Michael Gelinsky/ 12th June 2021
3D bioprinting of hepatocytes: core–shell structured co-cultures with fibroblasts for enhanced functionality scientific reports Rania Taymour, David Kilian, Tilman Ahlfeld, Michael Gelinsky & Anja Lode/ 4th March 2021
Bioprinting of Magnetically Deformable Scaffolds ACS Biomater. Sci. Eng. Janina Spangenberg, David Kilian, Charis Czichy, Tilman Ahlfeld, Anja Lode, Stefan Günther, Stefan Odenbach, and Michael Gelinsky/ 28th January 2021
Biomimetic Tympanic Membrane Replacement Made by Melt Electrowriting Advanced Healthcare Materials Max von Witzleben, Thomas Stoppe, Tilman Ahlfeld, Anne Bernhardt, Marie-Luise Polk, Matthias Bornitz, Marcus Neudert, and Michael Gelinsky/ 27th January 2021
Toward Biofabrication of Resorbable Implants Consisting of a Calcium Phosphate Cement and Fibrin—A Characterization In Vitro and In Vivo International Journal of Molecular Sciences Tilman Ahlfeld, Anja Lode, Richard Frank Richter, Winnie Pradel, Adrian Franke, Martina Rauner, Bernd Stadlinger, Günter Lauer, Michael Gelinsky, Paula Korn/ 26th January 2021
Three-Dimensional Melt-Electrowritten Polycaprolactone/Chitosan Scaffolds Enhance Mesenchymal Stem Cell Behavior ASC Publications Minami Yoshida, Paul R. Turner, M. Azam Ali, and Jaydee D. Cabral/ 13th January 2021
Melt electrowitten sandwich scaffold technique using Sulforhodamine B to monitor stem cell behavior Tissue Engineering Part C: Methods Dr. Paul R Turner, Miss Minami Yoshida, Dr. Azam Ali, and Dr. Jaydee Dones Cabral/ 19th Ocotber 2020
Peptide chitosan/dextran core/shell vascularized 3D constructs for wound healing ACS Publications Paul R. Turner, Eoin Murray, C. John McAdam, Michelle A. McConnell, and Jaydee Dones Cabral/ 28th June 2020
A hybrid additive manufacturing platform to create bulk and surface composition gradients on scaffolds for tissue regeneration bioRxiv Ravi Sinha, Maria Cámara-Torres, Paolo Scopece, Emanuele Verga Falzacappa, Alessandro Patelli, Lorenzo Moroni, Carlos Mota/ 23rd June 2020
3D Bioprinting of osteochondral tissue substitutes – in vitro-chondrogenesis in multi-layered mineralized constructs scientific reports David Kilian, Tilman Ahlfeld , Ashwini Rahul Akkineni, Anne Bernhardt, Michael Gelinsky & Anja Lode/ 19th May 2020
3D Printing of Bone Grafts for Cleft Alveolar Osteoplasty – In vivo Evaluation in a Preclinical Model frontiers in Bioengineering and Biotechnology Paula Korn, Tilman Ahlfeld, Franziska Lahmeyer, David Kilian, Philipp Sembdner, Ralph Stelzer, Winnie Pradel, Adrian Franke, Martina Rauner, Ursula Range, Bernd Stadlinger, Anja Lode, Günter Lauer and Michael Gelinsky/ 22nd March 2020
Engineering considerations on extrusion-based bioprinting: interactions of material behavior, mechanical forces and cells in the printing needle IOP Science Julia Emmermacher, David Spura, Jasmina Cziommer, David Kilian, Tobias Wollborn, Udo Fritsching, Juliane Steingroewer, Thomas Walther, Michael Gelinsky and Anja Lode/  11th March 2020
A Novel Plasma-Based Bioink Stimulates Cell Proliferation and Differentiation in Bioprinted, Mineralized Constructs ASC Publications Tilman Ahlfeld, Nieves Cubo-Mateo, Silvia Cometta, Vera Guduric, Corina Vater, Anne Bernhardt, A. Rahul Akkineni, Anja Lode, Michael Gelinsky/ 24th February 2020
3D bioprinted endometrial stem cells on melt electrospun poly ε-caprolactone mesh for pelvic floor application promote anti-inflammatory responses in mice Acta Biomaterialia Kallyanashis Paul, Saeedeh Darzia, Gordon Mc Phee, Mark P.Del, Borgod Jerome,  A. Werkmeister, Caroline E. Gargett, Shayanti Mukherjeeab/ August 2019
Investigating the effect of sterilisation methods on the physical properties and cytocompatibility of methyl cellulose used in combination with alginate for 3D-bioplotting of chondrocytes Journal of Materials Science Ella Hodder, Sarah Duin, David Kilian, Tilman Ahlfeld, Julia Seidel, Carsten Nachtigall, Peter Bush, Derek Covill, Michael Gelinsky, Anja Lode/ Jan 2019
3D Bioprinting of Functional Islets of Langerhans in an Alginate/Methylcellulose Hydrogel Blend Wiley Online Library Sarah Duin, Kathleen Schütz, Tilman Ahlfeld, Susann Lehmann, Anja Lode, Barbara Ludwig, Michael Gelinsky/ March 2019
3D Plotted Biphasic Bone Scaffolds for Growth Factor Delivery: Biological Characterization In Vitro and In Vivo Wiley Online Library Tilman Ahlfeld, Felix Paul Schuster, Yvonne Förster, Mandy Quade, Ashwini Rahul Akkineni, Claudia Rentsch, Stefan Rammelt, Michael Gelinsky, Anja Lode/ March 2019
A Methylcellulose Hydrogel as Support for 3D Plotting of Complex Shaped Calcium Phosphate Scaffolds Gels 2018 T. Ahlfeld, T. Köhler, Ch. Czichy, A. Lode, M. Gelinsky/ August 2018
Green bioprinting: extrusion-based fabrication of plant cell-laden biopolymer hydrogel scaffolds IOP Science J. Seidel, T. Ahlfeld, M. Adolph, S. Kümmritz, J. Steingroewer, F. Krujatz, T. Bley, M. Gelinsky and A. Lode/ Nov. 2017
Development of a clay based bioink for 3D cell printing for skeletal application IOP Science T. Ahlfeld, G. Cidonio D. Kilian, S. Duin, A. R. Akkineni, J. I. Dawson, S. Yang, A. Lode, R. O. C. Oreffo and M. Gelinsky/ July 2017
Fabrication of Cell-Loaded Two-Phase 3D Constructs for Tissue Engineering materials (Full Text) Tobias Zehnder, Tim Freund, Merve Demir, Rainer Detsch and Aldo R. Boccaccini/ Nov 2016
A versatile method for combining different biopolymers in a core/shell fashion by 3D plotting to achieve mechanically robust constructs IOP Publishing Ltd. Ashwini Rahul Akkineni, Tilman Ahlfeld, Anja Lode and Michael Gelinsky/ Oct 2016
In situ functionalization of scaffolds during
extrusion-based 3D plotting using a piezoelectric nanoliter pipette
Future Medicine Stefan Giron, Anja Lode, Michael Gelinsky/ Oct 2016
3D plotting of highly uniform Sr5(PO4)2SiO4 bioceramic scaffolds for bone tissue engineering Journal of Materials Chemistry Huiying Zhu, Dong Zhai, Chucheng Lin, Yali Zhang, Zhiguang Huan, Jiang Chang and Chengtie Wu/ Aug 2016
Evaluation of cell inkjet printing technique for biofabrication DE GRUYTER Rainer Detsch, Sebastian Blob, Tobias Zehnder and Aldo R. Boccaccini/ July 2016
Biofabrication of 3D Alginate-Based Hydrogel for Cancer Research: Comparison of Cell Spreading, Viability, and Adhesion Characteristics of Colorectal HCT116 Tumor Cells Mary Ann Liebert, Inc. Ivanovska Jelena, Zehnder Tobias, Lennert Pablo, Sarker Bapi, Boccaccini Aldo R., Hartmann Arndt, Schneider-Stock Regine, and Detsch Rainer/ July 2016
Design and Fabrication of Complex Scaffolds for Bone Defect Healing: Combined 3D Plotting of a Calcium Phosphate Cement and a Growth Factor-Loaded Hydrogel Annals of Biomedical Engineering Tilman Ahlfeld, Aswini Rahul Akkineni, Yvonne Foerster, Tino Koehler, Sven Knaack, Michael Gelinsky, and Anja Lode/ June 2016
A Hydrogel Model Incorporating 3D-Plotted Hydroxyapatite for Osteochondral Tissue Engineering materials (Full Text) Michal Bartnikowski, Ashwini Rahul Akkineni, Michael Gelinsky, Maria A. Woodruff, Travis J. Klein/ April 2016
Highly Concentrated Alginate-Gellan Gum Composites for 3D Plotting of Complex Tissue Engineering Scaffolds Polymers Ashwini Rahul Akkineni, Tilman Ahlfeld, Alexander Funk, Anja Waske, Anja Lode and
Michael Gelinsky/ April 2016
Additive manufacturing of collagen scaffolds by three-dimensional plotting of highly viscous dispersions IOP Publishing Ltd. Anja Lode, Michael Meyer, Sophie Brüggemeier, Birgit Paul, Hagen Baltzer, Michaela Schröpfer, Claudia Winkelmann, Frank Sonntag and Michael Gelinsky/ February 2016
Three-Dimensional Printing of Hollow-Struts-Packed Bioceramic Scaffolds for Bone Regeneration ACS Publications Yongxiang Luo, Dong Zhai, Zhiguang Huan, Haibo Zhu, Lunguo Xia, Jiang Chang, and Chengtie Wu/ November 2015
Three-dimensional plotted hydroxyapatite scaffolds with predefined architecture: comparison of stabilization by alginate cross-linking versus sintering SAGE Journals Alok Kumar, Ashwini R Akkineni, Bikramjit Basu and Michael Gelinsky/ November 2015
Concentrated gelatin/alginate composites for fabrication of predesigned scaffolds with a favorable cell response by 3D plotting RSC Advances Yongxiang Luo, Anja Lode, Ashwini Rahul Akkinenia and Michael Gelinsky/ May 2015
Alginate/Nanohydroxyapatite Scaffolds with Designed Core/Shell Structures Fabricated by 3D Plotting and in Situ Mineralization for Bone Tissue Engineering ACS Publications Yongxiang Luo, Anja Lode, Chengtie Wu, Jiang Chang, and Michael Gelinsky/ March 2015
Microarraying/ Liquid Handling Access Authors/ Publication Date
Controlling Surface Wettability for Automated In Situ Array Synthesis and Direct Bioscreening Wiley Advanced Materials Weilin Lin, Shanil Gandhi, Alan Rodrigo Oviedo Lara, Alvin K. Thomas, Ralf Helbig,
and Yixin Zhang
High resolution bioprinting of multi-component hydrogels IOP Science Ralf Zimmermann, Christoph Hentschel, Felix Schrön, Denise Moedder, Teresa Büttner, Passant Atallah, Thomas Wegener, Thomas Gehring, Steffen Howitz, Uwe Freudenberg/ July 2019
Sulfated Glycosaminoglycans as Viral Decoy Receptors for Human Adenovirus Type 37 viruses Naresh Chandra, Yan Liu, Jing-Xia Liu, Lars Frängsmyr, Nian Wu, Lisete M Silva, Mona Lindström, Wengang Chai, Fatima Pedrosa Domellöf, Ten Feizi and Niklas Arnberg/ March 2019
3D printed microneedles for insulin skin delivery International Journal of Pharmaceutics Cristiane Patricia PissinatoPere, Sophia N.Economidou, GurpritLall, ClémentineZiraud, Joshua S.Boateng, Bruce D.Alexander, Dimitrios A.Lamprou, DennisDouroumis/ June 2018
Advances in Lectin Microarray Technology: Optimized Protocols for Piezoelectric Print Conditions Wiley Online: Current Protocols Kanoelani T. Pilobello, Praveen Agrawal, Richard Rouse, Lara K. Mahal/ 2013
Antibody colocalization microarray: a scalable technology for multiplex protein analysis in complex samples. Mol. Cell. Proteomics, in press Mol. Cell Proteomics Pla-Roca, M., Juncker, D. 2012
Clinical utility of serum autoantibodies detected by protein microarray in melanoma. Int. J. Proteomics 2011 Sabel, M.S., Liu, Y.,Lubman, D.M. 2011
Towards multiple readout application of plasmonic arrays Beilstein Journal of Nanotechnology Cialla, D., Weber, K. 2011
The living microarray: a high-throughput platform for measuring transcription dynamics in single cells. BMC Genomics Rajan, S., Djambazian, H., Dang, H.C.P., Sladek, R., Hudson, T.J./ 2011
Application of photonic crystal enhanced fluorescence to cancer biomarker microarrays. ACS analytical chemistry Huang, C.-S., Ruimin Tan, R., Zangar, R.C. 2011
Template-free structuring of colloidal hetero-monolayers by inkjet printing and particle floating RSC Soft Matter Retsch, M., Dostert, K.-H., Nett, S.K., Vogel, N., Gutmann, J.S., Jonas, U./ 2010
The identification of auto-antibodies in pancreatic cancer patient sera using a naturally fractionated Panc-1 cell line. IOS Cancer Biomarkers Patwa, T., Pal, M., Brand, R.E., Simeone, D.M., Lubman, D.M. 2010
Generation of Live-Cell Microarrays by means of DNA-directed immobilization of specific cell-surface ligands. WILEY Angewandte Chemie Schroeder, H., Ellinger, B., Becker, C.F.W., Waldmann, H., Niemeyer, C.M./ 2007
Screening of Glycosylation Patterns in Serum Using Natural Glycoprotein Microarrays and Multi-Lectin Fluorescence Detection ACS Publications Patwa, T., Zhao, J., Anderson, Michelle A., Lubman, D., 2006
Generation of High Density Protein Microarrays by Cell-free in Situ Expression of Unpurified PCR Products The ASBM Angenendt, P., Kreuzberger, J., Glökler, J., Hoheisel, J./ 2006
Peptide microarrays for the detection of molecular interactions in cellular signal transduction NCBI Stoevesandt, O,, Elbs, M., Köhler, K., Lellouch, AC., Fischer, R., André, T., Brock, R./ 2005
Micro-Contact Printing Access Authors/ Publication Date
Patterned Thermoresponsive Microgel Coatings for Noninvasive Processing of Adherent Cells ASC Publications Katja Uhlig, Thomas Wegener, Jian He, Michael Zeiser, Johannes Bookhold, Inna Dewald, Neus Godino, Magnus Jaeger, Thomas Hellweg, Andreas Fery, Claus Duschl/ Feb 2016
Donut-shaped chambers for analysis of biochemical processes at the cellular and subcellular levels Lab on a Chip N. Zurgil, O. Ravid-Hermesh, Y. Shafran, S. Howitz, E. Afrimzon, M. Sobolev, J. He, E. Shinar, R. Goldman-Levic and M. Deutsch/ July 2014
Nanoimprint patterning of thin cadmium stannate films using a polymeric precursor route RSC Publishing Benjamin Schumm, Philipp Wollmann, Julia Fritsch, Julia Grothe, Stefan Kaske, Journal of Materials Chemistry, Issue 29/ 2011
MicCell & Microfluidics Access Authors/ Publication Date
Plasmonic sensor for on-site detection of diclofenac molecules Sensors and Actuators B: Chemical Nadja Steinke, Sarah Döring, Roland Wuchrer, Christoph Kroha, Gerald Gerlach, Thomas Härtling/ June 2019
Portable and low-cost biosensor towards on-site detection of diclofenac in wastewater Talanta Schirmer, Posseckardt, Schröder, Gläser, Howitz, Scharff, Mertig/ May 2019
Optical and impedimetric study of genetically modified cells for diclofenac sensing Journal of Sensors and Sensor Systems Margarita Guenther, Falko Altenkirch, Kai Ostermann, Gerhard Rödel, Ingo Tobehn-Steinhäuser, Steffen Herbst, Stefan Görlandt, and Gerald Gerlach/ May 2019
Hydrogel-Based Plasmonic Sensor Substrate for the Detection of Ethanol Sensors 2019 Christoph Kroh , Roland Wuchrer, Nadja Steinke, Margarita Guenther, Gerald Gerlach  and Thomas Härtling/ March 2019
Encapsulating genetically modified Saccharomyces cerevisiae cells in a flow-through device towards the detection of diclofenac in wastewater Journal of Biotechnology C.Schirmera, J.Posseckardt, A.Kicka, K.Rebatscheka, W.Fichtner, K.Ostermann, A.Schuller, G.Rödel, M.Mertig/ Oct 2018
Donut-shaped chambers for analysis of biochemical processes at the cellular and subcellular levels Lab-on-a-Chip N. Zurgil, O. Ravid-Hermesh, Y. Shafran, S. Howitz, E. Afrimzon, M. Sobolev, J. He, E. Shinar, R. Goldman-Levic and M. Deutsch/ May 2014
Measuring acoustic energy density in microchannel acoustophoresis using a simple and rapid light-intensity method Lab-on-a-Chip Rune Barnkob, Ida Iranmanesh, Martin Wiklund and Henrik Bruus/ March 2012
The Role of Lipids in VDAC Oligomerization Biophysical Journal Viktoria Betaneli, Eugene P. Petrov, Petra Schwille/ February 2012