Bioinstruments
and
Microfluidics

Upcoming events/ trade shows:

GeSiM: Hall 1/ E18

GeSiM: Hall 1/ E18

  • We make instruments for microarray printing, 3D bioprinting, chemical synthesis, microfluidics and more.

  • Differently sized motion systems bridge the gap from research to production. All are smart, compact and highly accurate.

  • Tools delivering liquids, pastes, powders combine with handling tools for tubes and vessels. Tailored solutions are available on request.

  • Please ask for feasibility tests with your material: Customer service is a priority with us.

About us…

Application Spotlights / Customer Projects

Microarrayer Applications
Our Service for your Microarrays
Pre-sales feasibility tests, tailored instruments and after-sales service will take you to success. We accept your material, please contact us to arrange details

Manufacturing of array based diagnostics
The manufacturing of state-of-the-art diagnostic kits requires more precise liquid handling than ever before. The Nano-Plotter is one solution to address these needs. The instrument covers the volume range from Picolitre to Microlitre, well appropriated for but not limited to the classical microarray format. The flexible software prints lines on Nitrocellulose membranes and applies Nanolitre volumes to microfluidic cartridges.

Arraying into 96-well plates
Micro titer plate based assays allow more complex analysis when the well bottom is prepared with different well aligned capture molecules. Today commercial plates in the 96-well SBS format support reproducible tethering of proteins and other biomolecules to the plate bottom.

Large sample sets: Effective tip cleaning system of the Nano-Plotter avoids carry-over
The GeSiM Nano-Plotter keeps each sample species separated from the next one very precisely. Here we provide basic information on how to avoid carryover.

Reverse Phase Protein Arrays (RPPA)
A simple, robust and highly parallel assay architecture for monitoring of protein expression and activation. Our microarray printers are perfect to do lysed-cell arrays.

Glycoprotein arrays
Complementary to the popular antibody arrays, this application uses pre-separated proteins from cellular lysates or other biofluids. This work was conducted by the Lubman Research Laboratory, University of Michigan, Ann Arbor (USA).

Loading of Biosensors
Label free detection of biomolecules is an attractive solution for users, but challenges liquid handling technology. Small but exact sample aliquots needs to be tethered onto tiny areas of electronic chips. No problem for the Nano-Plotter camera system. Read how electrical biochips are manufactured at Campton Diagnostics.

Nanolitre Dosage Applications

Dispensing of Adhesives
Heatable piezoelectric dispensers enable micro dosage of Nanolitre volumes of high-viscous adhesives.

Collision of Droplets
The high kinetic energy of flying microdroplets supports mixing of mixable liquids when pre-dispensing mixing is not feasible.

µContactPrinter Applications

Fibronectin Patterns printed with µCP
Fibronectin coating is popular to enforce cell adhesion to surfaces. Microcontact printing allows partial and well defined preparation of surfaces. Here HUCB-NSC were grown on microcontact printed fibronectin squares.

Nanoplasmonic sensor structure by NIL
The performance of label-free SPR detection can be improved by nano-structured surfaces using NIL (Nano-Imprint Lithography). This customer project focuses  on the design of a waste-water sensor.

Picolitre wells for cell biology
NIL with UV-curable adhesives allows the design of Picolitre wells to attract single cells per well.

NIL of micro wells with interconnecting channels
Here we show NIL shaped microstructures for electrical activity measurement studies.

BioScaffolder Applications

Introduction
Here we present a general overview of bioprinter applications and printable materials for the GeSiM BioScaffolder instruments.

Melt Electro Writing of Milk Protein/PCL Blends for Skin Regeneration

The influence of milk proteins on the properties of PCL was investigated by the Department of Chemistry at the University of Otago, New Zealand. Special focus was put on the Melt Electro Writing unit of the GeSiM bioprinter BioScaffolder 3.1. The used milk proteins, Lactoferrin and Whey Protein, are supposed to increase the biological functionality of PCL.

PCL-PEG Blends for Tissue Engineering
A customer at Friedrich-Alexander University Erlangen (FAU) succeeded in improving the properties of PCL for tissue engineering by adding PEG. These biopolymers shall be optimized towards a quicker degradation/mass loss when getting in contact with body fluid.

Struts and Capsules
A group from the FAU Erlangen added prefabricated capsules enriched with cells to hydrogels before printing. Different components desirable for bone regeneration, e.g., cells and bioactive proteins, can be incorporated both in the capsules and struts. This enables compartmentalization of components, which facilitates greater flexibility in modification of the scaffold.

Green Bioprinting
A team from the Technische Universität Dresden printed algae cells suspended in hydrogel together with mammalian cells. The cocultivation of algae in close vicinity to human cells could enable a sustained delivery of oxygen or secondary metabolites with therapeutic potential to human cells without the need of external supply. The fabrication of patterned coculture scaffolds can be easily realized by two-channel plotting.

Alginate/Methylcellulose Blends
Immediate cross linking of alginate after printing can be avoided – Accordingly to an approach of researchers at the Technische Universität Dresden. The addition of Methylcellulose (MC) to low concentrated alginate leads to an enhanced viscosity and therefore improved printing conditions.

Bioprinting: The Future of Surgery?
In a joint research project with the Technische Universität Dresden a scaphoid bone scaffold model was printed from Calcium Phosphate Cement. As model the human scaphoid bone was selected and 3D data extracted from a CT scan.

As FAST as Possible
An EU funded Consortium (Horizon 2020) was setup to optimize bulk and surface properties of scaffold materials for implants. GeSiM has been engaged for the development of high-performance syringe extruders for printing of polymer gradients through a single tool and within one layer. Further GeSiM adapts the NADIR plasma pen onboard of the BioScaffold printer, it now synchronizes with all other GeSiM bioprinter tools

BioSyntheSizer Applications

Synthesis of Hydrogels

The BSyS 3.1/E (Large platform) was configured for the automatic synthesis of Hydrogels. Hydrogels are an important component of bioinks for tissue engineering.

MicCell Applications

Applications Overview
The MicCell is a versatile toolkit for doing optical transparent microfluidic prototypes. This section presents a list of already done applications and outlines the versatility of the MicCell system in life science research.

Wastewater Analysis Using Foil-Based Microfluidics
Beside of PDMS, laser cut foils allow to stack several microfluidic layers in a MicCell Setup. Here an autologous microfluidic system for waste water analysis was developed.

Foil based Multi-Layer Flow Cell to study Gradients
Membrane separated foil cut chips were used with a MicCell setup to exposes channel-bound collagen to different concentrations of cells.

Detection of Biological Polutants in Waste Water
A foil-based MicCell system was used for waste water analysis using genetically modified yeast cells.

Biofilms and Biofouling
Here the MicCell platform was used to develop antifouling coatings on transparant surfaces.

Microfluidics Applications

Foil-based Multi-Organ Chip
Customers developed a flow-cell in which overpressure or vacuum are exerted on cells or organoids, e.g. to monitor chondrocyte differentiation. These cells interfere with a robot (Microfluidic Workstation) for pipetting and optical measurement.

Silicon based Multi-Organ Chip
In cooperations with the Fraunhofer IWS Dresden a Multi-Bioreactor Chips was developed. It is supposed to enable studies of the interaction of consumer-relevant synthetic or natural substances with the human body in its typical environments and with its individual genotypic specificities.

GeSiM Company Brochure

Abstracts “20 Years GeSiM”

Welcome to GeSiM!

gesim-company

GeSiM is a leading supplier of instrumentation and services for low-volume liquid handling and microfluidics. Being in business since 1995 GeSiM focuses on off-the-shelf products more…